Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.146300 |
Chromosome: | chromosome 10 |
Location: | 207940 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g419150 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAACCTGCCTTACTCGTCGCCTCCAGGT |
Internal bar code: | CGCTGCGTCTGCAGGGAGGTCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 608 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 65 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGAAACCCGAACCTCACAT |
Suggested primer 2: | ACTGGAACTCGGTTTGGATG |