Insertion junction: LMJ.RY0402.146305_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.146305
Chromosome:chromosome 7
Location:5674768
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g352400 IPP5 Inositol polyphosphate related phosphatase; (1 of 2) 3.1.3.56 - Inositol-polyphosphate 5-phosphatase / Type I inositol-polyphosphate phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTGGAGGCGGCGCTGAGGGCGGTGGAC
Internal bar code:ATGAAGTGCCGCGGCCTAGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:325
LEAP-Seq percent confirming:96.3809
LEAP-Seq n confirming:3249
LEAP-Seq n nonconfirming:122
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTCACTCGACACGTGAA
Suggested primer 2:ATTAAGGACGCACCCACAAC