| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.146316 |
| Chromosome: | chromosome 4 |
| Location: | 2834705 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224200 | (1 of 2) PTHR18841//PTHR18841:SF0 - VITELLINE MEMBRANE OUTER LAYER PROTEIN I-RELATED // PROTEIN C08G5.3, ISOFORM A-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGGAGTTGTCAGCATGCCGGATGGTAT |
| Internal bar code: | GGACTGTGAGCCTCGCATCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 630 |
| LEAP-Seq percent confirming: | 95.6459 |
| LEAP-Seq n confirming: | 21286 |
| LEAP-Seq n nonconfirming: | 969 |
| LEAP-Seq n unique pos: | 105 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTTCTCAGAGGCTGGCT |
| Suggested primer 2: | GCACGGTACCCATGGTATTC |