Insertion junction: LMJ.RY0402.146333_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.146333
Chromosome:chromosome 13
Location:5070350
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g607100 CYG6 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED; Adenylate/guanylate cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTGTGAAGGGGCACACCTGACGCCTCC
Internal bar code:CGAGGCGTCGTCCCGCACCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:571
LEAP-Seq percent confirming:99.7993
LEAP-Seq n confirming:2984
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CATCTAATGATGCCACCACG
Suggested primer 2:CATTGCGTGTGAATTCTCGT