| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146355 |
| Chromosome: | chromosome_17 |
| Location: | 3958656 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g728650 | FAP196 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTGCGTGAGGGCAGCGCACACGTGCTGCA |
| Internal bar code: | CGAACAGCAGCCGGCGGCAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 76.4572 |
| LEAP-Seq n confirming: | 1679 |
| LEAP-Seq n nonconfirming: | 517 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGACGGGTCCATCTACG |
| Suggested primer 2: | TTTGGCTGATACCCGAAAAC |