| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.146364 |
| Chromosome: | chromosome 12 |
| Location: | 2645771 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAACCACTGCGTAGGCTAGCAACGCGTGC |
| Internal bar code: | GCGCCTTGGCTTTGGATGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 49 |
| LEAP-Seq percent confirming: | 99.6855 |
| LEAP-Seq n confirming: | 1268 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAACCTAACCCACATGC |
| Suggested primer 2: | CAGGTCTGGGAACTTGGTGT |