| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.146365 |
| Chromosome: | chromosome 17 |
| Location: | 4571516 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732900 | (1 of 1) IPR000104//IPR001126 - Antifreeze protein, type I // UmuC domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATCAACCCCCCGACGCTCCCTGCCTGG |
| Internal bar code: | TCGGGTACAGGTACAAATGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 315 |
| LEAP-Seq percent confirming: | 81.4301 |
| LEAP-Seq n confirming: | 763 |
| LEAP-Seq n nonconfirming: | 174 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGCGTTCAAAGTGGAGTA |
| Suggested primer 2: | TGCTGTAGTTGAGGTTGCGT |