Insertion junction: LMJ.RY0402.146369_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.146369
Chromosome:chromosome 16
Location:4511620
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g667350 CAV6 (1 of 2) PF00520//PF08016//PF16905 - Ion transport protein (Ion_trans) // Polycystin cation channel (PKD_channel) // Voltage-dependent L-type calcium channel, IQ-associated (GPHH); Voltage-gated Ca2+ channel, alpha subunit CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTGAACTTCATCTGCTCGTCTGCCTCGG
Internal bar code:AAGTGGTTTTTCCCTCATGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:627
LEAP-Seq percent confirming:99.5777
LEAP-Seq n confirming:1179
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CCACTGGTCATGGTCATCAG
Suggested primer 2:CTCTCTGGTCTCCTGCTGCT