| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.146371 |
| Chromosome: | chromosome 7 |
| Location: | 3347547 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334950 | (1 of 107) PF00069//PF07714 - Protein kinase domain (Pkinase) // Protein tyrosine kinase (Pkinase_Tyr) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGCCATGCGCACCGTGTGCCCACTACAC |
| Internal bar code: | AGCGTTTAGTGACAACGATACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1225 |
| LEAP-Seq percent confirming: | 99.3023 |
| LEAP-Seq n confirming: | 1708 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCCTCCAAACTTCACTC |
| Suggested primer 2: | CGTGGTGTTATGTACGTGGC |