| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146382 |
| Chromosome: | chromosome 6 |
| Location: | 5184906 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281700 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAAGTTGCTGAAGCAGCGGGTTCTTTCA |
| Internal bar code: | AACGGCGGCGAGGATTCCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 80.6061 |
| LEAP-Seq n confirming: | 1064 |
| LEAP-Seq n nonconfirming: | 256 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAACCGACGATGAAAGT |
| Suggested primer 2: | GAGCTGAAGGACGACCTGAC |