| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.146417 |
| Chromosome: | chromosome 16 |
| Location: | 7088618 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679445 | FLA1,FAS6 | (1 of 10) PTHR10900 - PERIOSTIN-RELATED; Fasciclin-like protein 6 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGCCTGTGACTGCCGCCCCGTCCCCCAT |
| Internal bar code: | CGCGGGAGGTCGGAGAACCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 514 |
| LEAP-Seq percent confirming: | 99.6381 |
| LEAP-Seq n confirming: | 826 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTAAAAGTAGCACGCAC |
| Suggested primer 2: | AACCAATGAGCGAGGATTTG |