| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.146443 |
| Chromosome: | chromosome 3 |
| Location: | 7214508 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207800 | ADH5,CAD1 | Cinnamyl-alcohol dehydrogenase; (1 of 2) 1.1.1.195 - Cinnamyl-alcohol dehydrogenase / CAD | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATTTCTGTGGATGGGAATGAGAGTCGG |
| Internal bar code: | TCTAATGTCTGGTTCCCGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 145 |
| LEAP-Seq percent confirming: | 98.977 |
| LEAP-Seq n confirming: | 1161 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCTGCAAGCCAAGAACT |
| Suggested primer 2: | AACCTGTAGACGGTGATGCC |