| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146459 |
| Chromosome: | chromosome 13 |
| Location: | 1550886 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572950 | E2F2,E2FR1 | Transcription factor, E2F and DP-related; (1 of 2) PTHR12548//PTHR12548:SF9 - TRANSCRIPTION FACTOR DP // TRANSCRIPTION FACTOR DP | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATGTGAAACGTATGTCCGCTTGTGAAAT |
| Internal bar code: | ATGCGGGTGCCCCTTCGCCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 941 |
| LEAP-Seq percent confirming: | 99.6331 |
| LEAP-Seq n confirming: | 3259 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCCCATCACATGGAAAT |
| Suggested primer 2: | TGGTGCTGTTTTGTGTCCAT |