| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.146461 |
| Chromosome: | chromosome 3 |
| Location: | 3585723 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168300 | (1 of 1) 3.4.21.107//3.4.21.108 - Peptidase Do / Protease Do // HtrA2 peptidase / Serine proteinase OMI | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGGGCTGAGGGAGAAGCGACCTGAAGCG |
| Internal bar code: | GAAAACGTACGCACAAATCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 852 |
| LEAP-Seq percent confirming: | 99.43 |
| LEAP-Seq n confirming: | 3663 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAACCAGAGGGAGATGGG |
| Suggested primer 2: | GAGTGGCTGAGTGGGCTAAG |