| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.146467 |
| Chromosome: | chromosome 16 |
| Location: | 7314464 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTGTCAATGGTATGAACAAGCGCACCC |
| Internal bar code: | TTTTAATTGACGCCTTGTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 99.6098 |
| LEAP-Seq n confirming: | 2042 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCATCTAGGTCGTCCACCG |
| Suggested primer 2: | CCTCAAGTTGCTCCAAAAGC |