| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.146489 |
| Chromosome: | chromosome 2 |
| Location: | 3084317 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095053 | 5'UTR_intron|outside_mRNA | ||
| Cre02.g095054 | 3'UTR_intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCGGTACAGCCAACAAGCCCGCCAACAA |
| Internal bar code: | ACATCGATGGGAGTTCGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1052 |
| LEAP-Seq percent confirming: | 97.9691 |
| LEAP-Seq n confirming: | 1013 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAACATCCAAAGGGTAGCA |
| Suggested primer 2: | TTCTTAGCGTTGCCCTCAGT |