| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146527 |
| Chromosome: | chromosome 4 |
| Location: | 2703146 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223375 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCTTCACGAGATGGCAATACCAGTGTC |
| Internal bar code: | TCGGGCTCCTACCGGGGGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 705 |
| LEAP-Seq percent confirming: | 88.3764 |
| LEAP-Seq n confirming: | 4273 |
| LEAP-Seq n nonconfirming: | 562 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTAATTCATCACTCCGCC |
| Suggested primer 2: | ATCACACGCGACAACGATAC |