| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146533 |
| Chromosome: | chromosome 7 |
| Location: | 62081 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g312500 | MITC4 | putative mitochondrial carrier protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCCGCCCCAGCGGCAATCGTGACAAGT |
| Internal bar code: | ACGCAGCGCGACTGCTAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 882 |
| LEAP-Seq percent confirming: | 99.5426 |
| LEAP-Seq n confirming: | 2829 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGACAGTGGTAAGGCAG |
| Suggested primer 2: | GACTCCCTAAGTTTGCAGCG |