Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.146536 |
Chromosome: | chromosome_9 |
Location: | 4154140 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g393954 | FAP106 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACAAATGAGCGTGCTGGCTGGTGTGAATTA |
Internal bar code: | GGTTGGGACGAGGATCATTGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 575 |
LEAP-Seq percent confirming: | 99.9076 |
LEAP-Seq n confirming: | 2162 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGTTGAGTGTGGACAGTGCT |
Suggested primer 2: | CTATTGGTGTGCGTGTGGTC |