Insertion junction: LMJ.RY0402.146577_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.146577
Chromosome:chromosome 1
Location:1968008
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g010650 (1 of 1) PF12530 - Protein of unknown function (DUF3730) (DUF3730) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCCACGCCGCGTCCAGCACCGCCGCCGC
Internal bar code:GGGAGGAGACGAGACGACCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:345
LEAP-Seq percent confirming:46.4789
LEAP-Seq n confirming:165
LEAP-Seq n nonconfirming:190
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTACAACTGATGCGGAA
Suggested primer 2:GTGCAAATGTGTGTGTGCAA