Insertion junction: LMJ.RY0402.146582_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.146582
Chromosome:chromosome 13
Location:2102366
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g577550 DDB2 (1 of 1) K10140 - DNA damage-binding protein 2 (DDB2); UV-damaged DNA binding complex subunit 2 protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGATTAGCTGTCGGTTGCCACCGACATT
Internal bar code:TCGACCTCCAATTCATAATCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:668
LEAP-Seq percent confirming:99.3808
LEAP-Seq n confirming:642
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGTAGATGTGGCAGCGTCCT
Suggested primer 2:TTTGACGACGATGACTGAGC