| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146586 |
| Chromosome: | chromosome 13 |
| Location: | 1633622 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573650 | SRS8,SRS5 | Serine/arginine-rich pre-mRNA splicing factor; (1 of 89) PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) (RRM_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCCCGGCCCCGGTCCCGGTCTGGGGACC |
| Internal bar code: | GGTGACTCGATGGTTGGACCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 99.555 |
| LEAP-Seq n confirming: | 9396 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCCTCCTCCTCTTCCTC |
| Suggested primer 2: | GTGTGGCTGCTCTAGAAGGG |