| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.146608 |
| Chromosome: | chromosome 9 |
| Location: | 6833364 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409750 | CAX2 | (1 of 3) K07300 - Ca2+:H+ antiporter (chaA, CAX); CAX family cation antiporter, membrane protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGAGGAATGCGATACATCGGAGAACTG |
| Internal bar code: | TGCCTTGTGGGTCATCGCAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 99.4558 |
| LEAP-Seq n confirming: | 731 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGATACGAGAAGACATGA |
| Suggested primer 2: | CCTCCAACACTCTAGCCAGC |