Insertion junction: LMJ.RY0402.146642_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.146642
Chromosome:chromosome_8
Location:4151438
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre08.g379900 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CCGTACGCTCGTCACCGTACGACAGAGATC
Internal bar code:AAAATGCGAAATAAGCGTTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:917
LEAP-Seq percent confirming:99.8752
LEAP-Seq n confirming:800
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTCCTGTGCTTTCCTGCT
Suggested primer 2:TTGTAACGACAGCTTGCGAC