Insertion junction: LMJ.RY0402.146666_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.146666
Chromosome:chromosome 16
Location:4673528
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g687301 SRH26 (1 of 1) K15192 - TATA-binding protein-associated factor [EC:3.6.4.-] (BTAF1, MOT1); SNF2-related DNA/RNA helicase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCTGCCAACCCTGCTACGACATGAGTAT
Internal bar code:CTGAGGGGCTTCTTGCGAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:357
LEAP-Seq percent confirming:97.455
LEAP-Seq n confirming:2489
LEAP-Seq n nonconfirming:65
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACCCAGGTGAACATCA
Suggested primer 2:CTCCATGCTTCCCTCTGAAG