| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146674 |
| Chromosome: | chromosome 3 |
| Location: | 5205144 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182650 | PGA1 | Phospholipid/glycerol acyltransferase; (1 of 2) K13511 - monolysocardiolipin acyltransferase (TAZ) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTGGTCATCCCTTACACGTGCACAGAT |
| Internal bar code: | GTTTTTCGGCATGGGCTCTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 227 |
| LEAP-Seq percent confirming: | 62.3234 |
| LEAP-Seq n confirming: | 1191 |
| LEAP-Seq n nonconfirming: | 720 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTTGCCTTACGGGATTG |
| Suggested primer 2: | TATGAAAGGCAAGTCCCTGG |