Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.146674 |
Chromosome: | chromosome_3 |
Location: | 5205144 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g182650 | PGA1 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTCTTGGTCATCCCTTACACGTGCACAGAT |
Internal bar code: | GTTTTTCGGCATGGGCTCTTTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 227 |
LEAP-Seq percent confirming: | 62.3234 |
LEAP-Seq n confirming: | 1191 |
LEAP-Seq n nonconfirming: | 720 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTGTTGCCTTACGGGATTG |
Suggested primer 2: | TATGAAAGGCAAGTCCCTGG |