Insertion junction: LMJ.RY0402.146682_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.146682
Chromosome:chromosome 3
Location:5900647
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g190000 PPP14 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGAACGCAATTGCCTCCTGCACCCGTG
Internal bar code:CAGGCGCCGTCTTTTGCACTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:881
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:96
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACAGTTCAAGGCGATCA
Suggested primer 2:CGGTTCCATGTCTGTTGTTG