| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.146706 |
| Chromosome: | chromosome 13 |
| Location: | 4753535 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g604950 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGTCGGGCCCCGCAGCCTAAGGCGGGC |
| Internal bar code: | TACGGCGGCTGCGAAACACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 610 |
| LEAP-Seq percent confirming: | 93.9759 |
| LEAP-Seq n confirming: | 2262 |
| LEAP-Seq n nonconfirming: | 145 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACCCTACGGGACTTTAC |
| Suggested primer 2: | TTTTGTAACTGCAGCCATGC |