| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.146727 |
| Chromosome: | chromosome 3 |
| Location: | 3973873 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171800 | FAP123 | Flagellar Associated Protein 123 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTACCGGGGCCTCGACCCAGCGTTGTTG |
| Internal bar code: | TTGTACAGCAGTACCCCGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 839 |
| LEAP-Seq percent confirming: | 99.7015 |
| LEAP-Seq n confirming: | 2004 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGATAGCTATGCACGCAA |
| Suggested primer 2: | ACACGAAACCGAATGACACA |