| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.146730 |
| Chromosome: | chromosome 14 |
| Location: | 3991764 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633300 | (1 of 1) IPR003582//IPR006626//IPR009030 - ShKT domain // Parallel beta-helix repeat // Insulin-like growth factor binding protein, N-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTACCACACCACCAATCAGGCATGGGCA |
| Internal bar code: | AGGCAATGGAGCTGCGGCTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 272 |
| LEAP-Seq percent confirming: | 92.6302 |
| LEAP-Seq n confirming: | 1458 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGTAGCGAGAACAGCGG |
| Suggested primer 2: | CTACTGCTACTGCCCTTGGC |