Insertion junction: LMJ.RY0402.146735_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.146735
Chromosome:chromosome 17
Location:4336406
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g731050 (1 of 1) PTHR12847//PTHR12847:SF5 - ATP-BINDING CASSETTE ABC TRANSPORTER-RELATED // CCR4-ASSOCIATED FACTOR 16 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTCATTCCGCAAATTGCCTAGGGCGCTC
Internal bar code:TAAGGCATGACCGTTGTTATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:939
LEAP-Seq percent confirming:99.0654
LEAP-Seq n confirming:106
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACAATCTGAGGGAACCGTTG
Suggested primer 2:CATCTCGCTTCAAGTCCCTC