| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.146741 |
| Chromosome: | chromosome_10 |
| Location: | 1835095 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g431150 | ROC22,FAP256 | Flagellar Associated Protein, involved in circadian rhythms | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGTGTCACGGAACAGCCAGGTGTGTTGCG |
| Internal bar code: | AATGAGGGAAATTCCGATGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 99.7636 |
| LEAP-Seq n confirming: | 422 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTACTGCTGCTACTGC |
| Suggested primer 2: | TTGATACTCATGCCAGCGAG |