| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146780 |
| Chromosome: | chromosome 10 |
| Location: | 530126 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421250 | EXO70 | (1 of 1) K07195 - exocyst complex component 7 (EXOC7, EXO70); Component of the Exocyst Complex | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCAGGTGTGCCGGCGTCCACGCAGAGC |
| Internal bar code: | TCATGGACTCCATGCGGACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 97.9167 |
| LEAP-Seq n confirming: | 235 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCGTGATTGTGTACCCT |
| Suggested primer 2: | AATGAAATGGGCACGAGAAC |