Insertion junction: LMJ.RY0402.146789_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.146789
Chromosome:chromosome_14
Location:866263
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre14.g613400 POC16 Centriole proteome protein sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):AGTAAAAACACGACAGGACAGGACGAGGAC
Internal bar code:AAATAACGAGAAAGAGTGTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:368
LEAP-Seq percent confirming:99.6212
LEAP-Seq n confirming:526
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCTCTTTAACACACGCCT
Suggested primer 2:TCGACTACCGTACTGGGACC