| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146790 |
| Chromosome: | chromosome 1 |
| Location: | 2114915 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCAATCGGCCACACGCTCTCTCGTACCG |
| Internal bar code: | CGCTCAAAGGTCGGCACAGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 99.309 |
| LEAP-Seq n confirming: | 1006 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGACCGAACACAAACAAGG |
| Suggested primer 2: | GACGTAACTCTGGTGGGGAA |