| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146792 |
| Chromosome: | chromosome 5 |
| Location: | 2859659 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237870 | 5'UTR_intron | ||
| Cre05.g237890 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAGCATGGGCGAGGGCATGGTATACGT |
| Internal bar code: | TCTATGGCGGGCTTAACTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 99.6066 |
| LEAP-Seq n confirming: | 1519 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACATGTCAGCCAAGAGCA |
| Suggested primer 2: | ATATCACGACACACAGGGCA |