| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.146817 |
| Chromosome: | chromosome 17 |
| Location: | 5671873 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738600 | RLS5 | RegA/Rls-like protein; (1 of 10) IPR010919 - SAND domain-like | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACAACAACACACACGGCCTCGTGCCTT |
| Internal bar code: | CAAACCATCTGCCTTCGTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 342 |
| LEAP-Seq percent confirming: | 99.2894 |
| LEAP-Seq n confirming: | 5030 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGGAAAGGAACAGGTCAA |
| Suggested primer 2: | AAATCAAGTTCGGTTTTGCG |