| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.146827 |
| Chromosome: | chromosome 14 |
| Location: | 413601 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610663 | (1 of 1) PTHR12801//PTHR12801:SF51 - EXONUCLEASE // PROTEIN Y56A3A.33 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCTGAGGGGTGCCGAGCTTGCGTTGCTT |
| Internal bar code: | ACTAATGTAGGTTGGCCTAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 99.6569 |
| LEAP-Seq n confirming: | 11618 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCGCGTAATGTACTGAAT |
| Suggested primer 2: | TGTGTGTGTGTGTCCTGGTG |