| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.146858 |
| Chromosome: | chromosome 3 |
| Location: | 8420563 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199199 | (1 of 20) IPR010095//IPR013083 - Transposase IS605, OrfB, C-terminal // Zinc finger, RING/FYVE/PHD-type | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTTGTACGGTGGGAGGGGGGAGGGGTC |
| Internal bar code: | TTCAATGCATCGTGCCCGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 99.7024 |
| LEAP-Seq n confirming: | 670 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAATCTGTCTAGCATGCCG |
| Suggested primer 2: | GTTGACGAATTGTGTGTCCG |