| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.146868 |
| Chromosome: | chromosome_6 |
| Location: | 7021036 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g296850 | FAP81 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCACCCGCAGGGTCCCAAGCTGTCGAGGTA |
| Internal bar code: | GTGGGGGCCGTCAACAGGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 77 |
| LEAP-Seq percent confirming: | 99.5098 |
| LEAP-Seq n confirming: | 609 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGACCCTCTACCTCACCA |
| Suggested primer 2: | CCCTTTACCTTGACGTGCAT |