Insertion junction: LMJ.RY0402.146868_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.146868
Chromosome:chromosome 6
Location:7021036
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g296850 FAP81,DLEC1 (1 of 8) IPR008962 - PapD-like; Flagellar Associated Protein 81 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCCGCAGGGTCCCAAGCTGTCGAGGTA
Internal bar code:GTGGGGGCCGTCAACAGGGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:77
LEAP-Seq percent confirming:99.5098
LEAP-Seq n confirming:609
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGACCCTCTACCTCACCA
Suggested primer 2:CCCTTTACCTTGACGTGCAT