| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.146884 |
| Chromosome: | chromosome 9 |
| Location: | 528557 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404000 | CPLD59 | (1 of 1) PF00805 - Pentapeptide repeats (8 copies) (Pentapeptide); thylakoid lumenal 17.4 kDa protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAGCCAGTGGACATAAAGCCAGCGGATC |
| Internal bar code: | CTTCGACCCACTCGTCCGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 932 |
| LEAP-Seq percent confirming: | 99.4951 |
| LEAP-Seq n confirming: | 2562 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTGAGCCTTTCATTCCT |
| Suggested primer 2: | GCGTAGCAAGAAGGGACAAG |