| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.146920 |
| Chromosome: | chromosome 10 |
| Location: | 711531 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422500 | (1 of 1) PF09113 - Peptide-N-glycosidase F, C terminal (N-glycanase_C) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTCACGCCCTGCCTGTGCCATGCCGCC |
| Internal bar code: | TAGCACTAGATCAGGGTTCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 505 |
| LEAP-Seq percent confirming: | 99.589 |
| LEAP-Seq n confirming: | 727 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGACTTCGAGATGCTGG |
| Suggested primer 2: | GCTGCTGAGCCTTTCAGTCT |