Insertion junction: LMJ.RY0402.146969_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.146969
Chromosome:chromosome 6
Location:6239851
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g290800 RMG1 RNA cap guanine-N2 methyltransferase; (1 of 1) 2.3.1.221 - Noranthrone synthase / Polyketide synthase A intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTACGACCCAACAGTTTCCTTTGGACAATG
Internal bar code:GTTTTGAGTGAATGCGAGGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:480
LEAP-Seq percent confirming:99.712
LEAP-Seq n confirming:12466
LEAP-Seq n nonconfirming:36
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGACAGATTGCCTTGACTGC
Suggested primer 2:TCTTCAGCAGCTCTTCCTCC