| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.146994 |
| Chromosome: | chromosome 2 |
| Location: | 5975164 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112150 | (1 of 10) 3.1.1.5 - Lysophospholipase / Phospholipase B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCGCCGAACATGTACAGGTGCAGATGC |
| Internal bar code: | TTTACGACAGCGCCGATTCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 801 |
| LEAP-Seq percent confirming: | 99.656 |
| LEAP-Seq n confirming: | 3187 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCCTGACAAAGCGCTAA |
| Suggested primer 2: | GTACAAGTCCCCAACTGCGT |