| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.146999 |
| Chromosome: | chromosome 12 |
| Location: | 5918063 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534151 | EEP9 | (1 of 3) PTHR22748:SF4 - DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE 2; endonuclease/exonuclease/phosphatase family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGATAGCCTTTTACCGGGTAAGAGCTGA |
| Internal bar code: | GGGATGGGGCCTTCAATATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 136 |
| LEAP-Seq percent confirming: | 96.9697 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCTCCAAAGAGCATTTC |
| Suggested primer 2: | TCCTTGAGGTCCTCCATGTC |