| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.147000 |
| Chromosome: | chromosome 11 |
| Location: | 1129244 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467680 | (1 of 13) 3.1.13.4 - Poly(A)-specific ribonuclease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACATCCTCGCTTGAAACACACACACAGC |
| Internal bar code: | GACGGAGCGCGTCGCGAGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 92.3453 |
| LEAP-Seq n confirming: | 6792 |
| LEAP-Seq n nonconfirming: | 563 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGGATCTCTTTTATGCAA |
| Suggested primer 2: | CCTTCACCTCCATCCACAAC |