| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147011 |
| Chromosome: | chromosome 3 |
| Location: | 3079318 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164600 | PMA3,PMH1,ACA3 | (1 of 3) K01535 - H+-transporting ATPase (E3.6.3.6); P-type ATPase/cation transporter, plasma membrane | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGTAATGCCATAGCCAAGACAGTGAGC |
| Internal bar code: | CCAGGGTCGGCTGTAAATATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 382 |
| LEAP-Seq percent confirming: | 99.3902 |
| LEAP-Seq n confirming: | 326 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGAGGAAATCTTTGCCAG |
| Suggested primer 2: | ACGACAATGTCACCTCCACA |