Insertion junction: LMJ.RY0402.147036_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.147036
Chromosome:chromosome 7
Location:2400083
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g328600 (1 of 1) K11804 - WD repeat-containing protein 42A (WDR42A) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGCCACGCTCCTAGACAGGTCCATGCAG
Internal bar code:GAGTGGAAGGTACGTAGCGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:305
LEAP-Seq percent confirming:58.934
LEAP-Seq n confirming:2134
LEAP-Seq n nonconfirming:1487
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TTTACCTGCTCGCCAGTCTT
Suggested primer 2:ACGAAGAAAGGTTGCGAAGA