Insertion junction: LMJ.RY0402.147036_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.147036
Chromosome:chromosome 7
Location:2400089
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g328600 (1 of 1) K11804 - WD repeat-containing protein 42A (WDR42A) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCATTCGAGCGACCCCTCCCTCCCTGCA
Internal bar code:CGCCATAAGGTAAGGGCCTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:434
LEAP-Seq percent confirming:81.6686
LEAP-Seq n confirming:4317
LEAP-Seq n nonconfirming:969
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:ACGAAGAAAGGTTGCGAAGA
Suggested primer 2:ACGTCACGTGTGTGGTGTTT