| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147045 |
| Chromosome: | chromosome_10 |
| Location: | 6510617 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g466650 | FAP252 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCCATAAACTTCGGCTACAAGGTTGTGCG |
| Internal bar code: | GGAGTCACACGATCGTGTCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1137 |
| LEAP-Seq percent confirming: | 99.5327 |
| LEAP-Seq n confirming: | 639 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTACCGTATGGTGCAGTT |
| Suggested primer 2: | CAGCACAACTCACTCTCCCA |